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发布于:2020-7-7 13:07:25  访问:25 次 回复:0 篇
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. The RNA-seq dataset was also accustomed to review the expression profiles
The B. rapa actin gene 81-88-9 Epigenetics Bra028615 was applied as an internal command. Primers for qRT-PCR ended up made employing Beacon Designer 7. The qRT-PCR experiments have been carried out with 3 organic and technological replicates. The SYBR Premix Ex Taq kit R (TaKaRa, Dalian, China) was accustomed to detect gene expression in accordance on the manufacturer‘s suggestions within the One-step Real-Time PCR Procedure Time PCR Detection Program (Applied Biosystems, Foster Town, CA, United states). The cycling profile was as follows: ninety four C for thirty s, 94 C for ten s for forty cycles, and fifty eight C for thirty s, followed by a melting curve evaluation at sixty five C for 10 s for 61 cycles. The relative gene expression degrees wereFrontiers in Plant Science | www.frontiersin.orgMarch 2017 | Volume eight | ArticleYan et al.Brassica rapa ABC Transporter FamilyTABLE 1 | Standard facts in regards to the ABC transporter gene household in B. rapa. Gene BraABCA1 BraABCA2 BraABCA3 BraABCA4 BraABCA5 BraABCA6 BraABCA7 37318-06-2 Purity BraABCA8 BraABCA9 BraABCA10 BraABCA11 BraABCB1 BraABCB2 BraABCB3 BraABCB4 BraABCB5 BraABCB6 BraABCB7 BraABCB8 BraABCB9 BraABCB10 BraABCB11 BraABCB12 BraABCB13 BraABCB14 BraABCB15 BraABCB16 BraABCB17 BraABCB18 BraABCB19 BraABCB20 BraABCB21 BraABCB22 BraABCB23 BraABCB24 BraABCB25 BraABCB26 BraABCB27 BraABCB28 BraABCB29 BraABCB30 BraABCB31 BraABCB32 BraABCB33 BraABCB34 BraABCB35 BraABCB36 BraABCB37 BraABCB38 BraABCC1 Locus title Bra033785 Bra000236 Bra012931 Bra012928 Bra012927 Bra018113 Bra018114 Bra018115 Bra018116 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25852654 Bra019513 Bra015049 Bra011043 Bra013936 Bra033043 Bra006776 Bra023087 Bra000136 Bra012621 Bra019135 Bra014756 Bra028221 Bra017216 Bra040475 Bra004484 Bra005036 Bra019907 Bra025425 Bra025359 Bra025331 Bra025328 Bra025326 Bra003445 568-72-9 Epigenetics Bra003950 Bra003490 Bra010464 Bra030503 Bra039056 Bra039055 Bra039042 Bra032864 Bra032856 Bra032855 Bra027534 Bra017539 Bra017540 Bra033331 Bra002664 Bra002094 Bra009500 Bra023893 Subfamily ABCA ABCA ABCA ABCA ABCA ABCA ABCA ABCA ABCA ABCA ABCA ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCB ABCC Chromosome A01 A03 A03 A03 A03 A06 A06 A06 A06 A06 A07 A01 A01 A02 A03 A03 A03 A03 A03 A04 A04 A04 A04 A05 A05 A06 A06 A06 A06 A06 A06 A07 A07 A07 A08 A08 A09 A09 A09 A09 A09 A09 A09 A09 A09 A10 A10 A10 A10 A01 Situation 14252485?4257069 10073306?0085122 21458462?1462337 21470698?1474908 21475917?1479405 10311197?0315562.. The RNA-seq dataset was also accustomed to assess the expression profiles of syntenic paralog pairs in different tissues. To analyze the expression styles of syntenic BraABC PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25852654 paralog pairs underneath normal and abiotic stress situations, the cultivar Chiifu-401-42 was cultivated in potting soil at 24/16 C, which has a photoperiod of 14/10 h for day/night within a progress chamber. Five-leaf-stage vegetation ended up utilized to perform distinct abiotic anxiety therapies underneath ongoing time (6, twelve, and 24 h). Hormone procedure was carried out with a hundred ABA; for drought pressure remedy, the pots were being irrigated with 15 (w/v) polyethylene glycol PEG and kept standing in the irrigation answer for thirty min underneath ordinary progress ailments; salt cure was performed with 250 mM NaCl.
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